The AmpliGrid Single Cell One-Step RT-PCR System has been designed for highly sensitive one-step RT-PCR reactions using single cell or low copy number RNA templates. The kit contains a hot-start DNA polymerase and a highly sensitive reverse transcriptase.
The kit provides highly specific reverse transcription and PCR on the AmpliGrid slide, using gene-specific primers on either total RNA or mRNA from single cell templates. The proprietary buffer is highly optimized and balanced, leading to outstanding results.
The single cell template amount should be in the range of 1 to 50 cells. When using isolated RNA, starting amounts from 5 pg to 1 ng have been tested. After cDNA synthesis has been performed, the reaction is heated to 95°C for 10 minutes to inactivate the RT enzymes, and simultaneously to activate the hot-start DNA polymerase.
Single Cell One-Step RT-PCR System
Pre-optimized reagents for single cell and 1 μL RT-PCR
- Pre-optimized for performance and compatibility with AmpliGrid single cell and 1 μL RT-PCR
- One-Step RT-PCR setup
- Master mix format to minimize hands-on time
- Optional on-slide DNase treatment or cell lysis with AmpliGrid Cell Extraction Kit for hard to lyse cells
