Example protocols for easy transfer of established methods to the AmpliGrid system.
- Quantitative RT-PCR on AmpliGrid AG480F
This process instruction describes the quantitative analysis of the housekeeping gene Calm. The reverse transcription is done on the AmpliGrid system using the Qiagen OneStep RT PCR Kit. Afterwards the quantitative amplification is done on a conventional real-time instrument (Stratagene) using SYBR green. (Download pdf / 293 KB) - Singleplex or Multiplex PCR of human DNA on AmpliGrid AG480F
In the singleplex PCR system, one DNA fragment is amplified using human template and the GoTaq® PCR kit or a Qiagen Kit on the AmpliGrid slides.
In the multiplex PCR system, four DNA fragments are amplified using female human DNA template and six DNA fragments.
(Download pdf / 229 KB) - Plasmid Amplification and Sequencing on AmpliGrid AG480F This protocol describes the amplification of plasmid DNA and the following sequencing reaction directly on the AmpliGrid Slide. (Download pdf / 235 KB)
- On-Slide DNA Sequencing using AmpliGrid AG480F
This protocol describes the sequencing procedure after PCR of human DNA directly on the AmpliGrid slide. (Download pdf / 211 KB) - RT-PCR on single mouse cells and extracted RNA using Qiagen reagents and AmpliGrid AG480F This process instruction describes a one-step RT-PCR for mouse B-lymphocytes and extracted total mouse RNA. In this RT-PCR system, b2m and Actin B fragments are amplified using the Qiagen OneStep RT-PCR kit together with AmpliGrid slides. (Download pdf / 249 KB)
- RT-PCR on single mouse cells and extracted RNA using AmpliGrid AG480F This process instruction describes a One-Step RT-PCR for mouse B-lymphocytes and extracted total mouse RNA. In this RT-PCR system, b2m and Actin B fragments are amplified using the Single Cell One-Step RT-PCR kit together with AmpliGrid slides. (Download pdf / 248 KB)
- RT-PCR on human total RNA and single cells using Qiagen reagents and AmpliGrid AG480F This process instruction describes a one-step RT-PCR for human lymphocytes or human total RNA. In this RT-PCR system, b2m and Calm fragments are amplified using the Qiagen OneStep RT-PCR kit together with AmpliGrid slides. (Download pdf / 246 KB)
- RT-PCR on human total RNA and single cells using AmpliGrid AG480F This process instruction describes a One-Step RT-PCR for human lymphocytes or human total RNA. In this RT-PCR system, b2m and Calm fragments are amplified using the Single Cell One-Step RT-PCR kit together with AmpliGrid slides. (Download pdf / 251 KB)
- Genetic Profiling with Applied Biosystems AmpFISTR® SEfiler™ on AmpliGrid AG480F
In this PCR system, 11 specific STR loci are amplified using human DNA as template and the AmpFISTR® SEfiler™ kit from Applied Biosystems together with the AmpliGrid slides. (Download pdf / 238 KB) - Genetic Profiling with Applied Biosystems AmpFISTR® Identifiler® on AmpliGrid AG480F In this PCR system, 15 specific STR loci and the gender-determining Amelogin are co-amplified using human DNA or cells as template and the AmpFISTR® Identifiler® kit from Applied Biosystems together with the AmpliGrid slides. (Download pdf / 235 KB)
- Genetic Profiling with Promega PowerPlex® 16 on AmpliGrid AG480F
This protocol describes the process of amplifying STR DNA fragments using the Promega PowerPlex® 16 System. (Download pdf / 240 KB) - Home-made AmpliGrid adaptor for non-automated P.A.L.M. LCM systems This protocol shows how to assemble a simple and affordable solution to fit AmpliGrid slides into an existing P.A.L.M. LCM system. (Download pdf / 1660 KB)
- TaqMan® microRNA Assay on single cells using AmpliGrid AG480F This process instruction describes an experiment using the TaqMan® microRNA Assay (Applied Biosystems) together with the AmpliGrid slide. (Download pdf / 259 KB)
- ELISA in 1 μL on AmpliGrid AG480E This protocol shows the detection of the target proteins MMP10 and MMP13
on the AmpliGrid AG480E using ELISA technique. (Download pdf / 509 KB) - Polyacrylamide Gel Electrophoresis & Silver Staining This process instruction characterizes the standard processing of a polyacrylamide gel electrophoresis and the consecutive silver staining. This instruction permanently ensures a constant quality of the analysis of PCR products. (Download pdf / 248 KB)
