Add single cell molecular analysis to flow cytometry
As a flow cytometry operator or user, you sort millions of cells by their physical and protein characteristics but have wondered how to add single cell genetic information to your analysis. Now the Advalytix product group of Beckman Coulter has developed a simple, affordable and reliable method to genetically characterize flow sorted cells. Available through a growing list of AmpliGrid-certified flow facilities (see list below).
• Workflow fully integrated with flow cytometry sorting
• Single cell sensitivity for PCR, rtPCR, qPCR, sequencing
• Saves reagent cost and sample material due to ultra-low, 1ul reaction volume
• Up to 100% success of depositing single cells – much better than sorting into MTPs
• Visual control of placement success
OUR SOLUTION for Flow cytometry
Our AmpliGrid system integrates with major flow cytometry sorters e.g., the BC MoFloTM. Simply place our AmpliGrid slide into your sorter’s MTP bay (see video), software-define the AmpliGrid, start sorting. We offer documentation, accessories and support to help you treat your flow cytometry sorted cells with single cell molecular analysis:
• Quick start guide: Step-by-step instructions for AmpliGrid sorting with flow cytometers BC MoFlo™, BD FACSAria™ and BD FACSVantage™.
• Slide adaptor: For flow cytometer improves precision, simplifies device set-up
• Calibration slide: simplifies calibration of your flow sorter’s aim
USER STORIES
• Dr. Rob Barrington differentiates sub-groups of extremely rare mouse memory B cells
• Toralf Kaiser uses CellEvator to prevent event rate drop-off and improves cell viability
• Read what Richard Konz and Ted Giehl have to say about the CellEvator
Looking for a flow core to sort YOUR cells onto AmpliGrid? Contact one of the following “AmpliGrid certified” flow cytometry facilities who have demonstrated >90% success rate of placing single cells into AmpliGrid:
AmpliGrid certified flow cytometry facilities
- Brigham & Women’s Hospital, Boston, MA
- UMass Medical School Center for Infectious Disease and Vaccine Research, Worcester, MA
- UMass Medical School, Worcester, MA
- Roswell Park Cancer Institute, Buffalo, NY
- Thomas Jefferson University Kimmel Cancer Center, Philadelphia, PA
- NIH’s National Heart, Lung, and Blood Institute, Bethesda, MD
- NIH’s National Cancer Institute, Bethesda, MD
- Indiana University Bloomington, IN
- University of Alabama at Birmingham, Birmingham, AL
- University of South Alabama, AL
- Creighton University, Omaha, NE
- MD Anderson Cancer Center, Houston, TX
- The Gladstone Institutes, San Francisco, CA
- Stanford University, Palo Alto, CA
- Feinstein Institute, Long Island, NY
- German Rheumatism Research Center, Berlin, Germany
- Helmholtz Association, Institute of molecular Immunology, Munich, Germany
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BD FACSVantage™ and BD FACSAria™ are registered trademarks of Becton, Dickinson and Company, USA
