AmpliCell
Cell Culture-to-PCR on one slide
- On-slide cell culture of adherent cells and cells in solution
- No cell preparation needed => minimized cellular stress
- in situ cell experiments to PCR in few simple steps
- Full visual control of workflow
- 4 PCR replicates per cell culture well
- Save up to 90 % reagent costs
- Compatible to HT systems (e.g., flow cytometry, robotics)
Transfection-to-PCR
Conventional transfection assays require large reagent volumes to ensure optimal growth conditions. Extraction adds to process time and cost. AmpliCell provides optimal growth conditions with only 100µl reagent volume which saves up to 90% reagent costs. No extraction is needed further reducing process time and costs. Workflows: (1) grow cells first then apply transfection reagent, (2) reverse approach: apply transfection reagent without prior cell culture to allow up to 3 runs per week. Take advantage of AmpliCell’s optical control of cell morphology and transfection efficiency prior to PCR: What you see is what you amplify!
AmpliCell is an open platform and for use with commercially available transfection chemistries. See the transfection application report here.
Methylation assay on the AmpliCell
Conventional methylation methods require multiple sample transfers which induces cell stress that can bias methylation results; they are also time consuming and costly. With AmpliCell, carry out complete cell growth, treatment and sequencing reaction workflow on the same substrate: (1) cultivate cells on AmpliCell and apply bisulfite treatment in a 1µL reaction, (2) visually control cells, (3) amplify gene(s) of interest and run sequencing reaction (no extraction needed), (4) remove amplicons to analyze results.
See the methylation application report here.
- Transfection assays with RNA / DNA
- microRNA assays
- in vivo gene silencing (siRNA assays)
- High throughput gene expression analysis
- Direct sequencing from cell populations
- High resolution fluorescent microscopy (IF)
